ChangeinHemoglobinLevelsduetoAnesthesiainMice3

The mean hemoglobin in group 1 was 9.9 mmol/L prior to the anesthesia and decreased to 8.7 mmol/L 10 min after administration of the anesthesia (p?<?0.001). At t?=?20, 30, and 45 min, it remained at approximately 8.9 but decreased further and was 7.9 mmol/L at termination of the study. Group 2, which received no anesthesia, remained at the same level of hemoglobin (mean 9.5 mmol/L) at t?=?10, 20, and 30 min but decreased slightly at t?=?45 min and fell to 9.1 mmol/L at t?=?105 min.

Discussion

Anesthesia induces physiological changes in animals as well as in humans. After administration of fentanyl or midazolam sedation, hypotension, bradycardia, vasodilatation, respiratory depression, and consequently hypoxia can occur (1, 4).

This study shows that the hemoglobin level is significantly lower in anesthetized animals compared to non-anesthetized animals. The difference is due to the administered drugs and not to the mice or the blood sampling procedure, since the curves cross each other when the anesthetized group becomes non-anesthetized and vice versa. Throughout the study, we have collected all blood samples from the same anatomical site knowing that also different anatomical locations can give different hemoglobin levels (5).

In the second part of the cross-over study, the difference between the anesthetized and the non-anesthetized groups seems bigger than the difference in the first part of the study. This can, at some points, be explained by mice that differ considerably in hemoglobin from the mean values as in blood sample 5, but another explanation could be an impact on the mouse of the repeated blood sampling. Even though there is 1 week between each sampling, some extent of extra bleeding might occur. The decline in both curves at blood sample 8 and 9 could be in accordance with this theory.

That the difference between anesthetized and non-anesthetized is bigger after the cross-over of the treatment could be due to group A being artificially high. During anesthesia, the mice get hypoxic, and this is known to induce an endogenous release of EPO (6). The main role of EPO is to stimulate the erythropoiesis in the bone marrow in a number of physiological conditions, and an increase in serum EPO has been observed 4 h after administration of Hypnorm/Dormicum to mice (1) (Gothelf et al., submitted for publication).

After induction of anesthesia, a decrease of approximately 1 mmol/L in hemoglobin occurs within 10 min and is not returned to the initial level within 30 min. Ceylan et al. (7) found a decrease in hemoglobin 30 min after intramuscular injection of tiletamin and zolazepam to sheep, but the level was normalized after 60 min. In the time-course study, the hemoglobin did not return to baseline within the 105 min the study lasted; on the contrary, the hemoglobin continued to decrease, and after 30 min, a decrease was also observed in the non-anesthetized group. This is probably due to the repeated blood sampling and handling of the animals.

The observed decrease in hemoglobin during anesthesia can be due to erythrocyte sequestration in the spleen (8), hypothermia, which is frequently occurring during anesthesia (9), or merely due to the analysis of hypoxic blood, but further studies are warranted in order to investigate the mechanism.

In this study, we have shown that anesthesia is an important confounder in the measurement of hemoglobin and must be taken into account when designing studies involving hemoglobin as a parameter.

By: Anita Gothelf1, Pernille Hojman1 2, Julie Gehl1 3

References

1. Whelan G, Flecknell PA (1994 Jan) The use of etorphine/methotrimeprazine and midazolam as an anaesthetic technique in laboratory rats and mice. Lab Anim 28(1):70–77

2. Hojman P, Gissel H, Gehl J (2007 Jun) Sensitive and precise regulation of haemoglobin after gene transfer of erythropoietin to muscle tissue using electroporation. Gene Ther 14(12):950–959

3. Hojman P, Brolin C, Gissel H, Brandt C, Zerahn B, Pedersen BK et al (2009) Erythropoietin over-expression protects against diet-induced obesity in mice through increased fat oxidation in muscles. PLoS ONE 4(6):e5894

4. Katzung BG (1992) Basic and clinical pharmacology, 5th edn. Appleton and Lange, East Norwalk

5. Nemzek JA, Bolgos GL, Williams BA, Remick DG (2001 Oct) Differences in normal values for murine white blood cell counts and other hematological parameters based on sampling site. Inflamm Res 50(10):523–527

6. Pagel H, Engel A, Jelkmann W (1992) Erythropoietin induction by hypoxia. A comparison of in vitro and in vivo experiments. Adv Exp Med Biol 317:515–519

7. Ceylan C, Aydilek N, Ipek H (2007 Jun) Effects of tiletamine-zolazepam anaesthesia on plasma antioxidative status and some haematological parameters in sheep. Acta Vet Hung 55(2):191–197

8. Wilson DV, Evans AT, Carpenter RA, Mullineaux DR (2004 Apr) The effect of four anesthetic protocols on splenic size in dogs. Vet Anaesth Analg 31(2):102–108

9. Grasshoff C, Drexler B, Rudolph U, Antkowiak B (2006) Anaesthetic drugs: linking molecular actions to clinical effects. Curr Pharm Des 12(28):3665–3679