AGuidetoCORNETfortheConstructionofCoexpressionandProteinProtein..
To enable easy access and interpretation of heterogenous and scattered data, we have developed a user-friendly tool for data mining and integration in Arabidopsis thaliana , designated CORrelation NETworks (acronym CORNET), allowing browsing of microarray data, construction of coexpression and protein–protein interactions (PPIs), analysis of gene association and transcription factor (TF) regulatory networ......閱讀全文
A－Guide－to－CORNET－for－the－Construction－of－Coexpression－and－ProteinProtein..
To enable easy access and interpretation of heterogenous and scattered data, we have developed a user-friendly tool for data mining and integratio
Complete－PCR－Guide
In the polymerase chain reaction (PCR), a thermostable DNA polymerase amplifies DNA that is flanked by known sequences. The known sequences correspond
PCR－Trouble－shooting－guide
1．假陰性，不出現擴增條帶 PCR反應的關鍵環節有①模板核酸的制備，②引物的質量與特異性，③酶的質量， ④PCR循環條件。尋找原因亦應針對上述環節進行分析研究。 模板：①模板中含有雜蛋白質，②模板中含有Taq酶抑制劑，③模板中蛋白質沒有消化除凈，特別是染色體中的組蛋白，④在提取制備模板時丟失過多，或
Degenerate－PCR,－a－short－guide.
What is degenerate PCR????Degenerate PCR is in most respect identical to ordinary PCR, but with one major difference. Instead of using specific PCR pr
Guide－to－Cell－Proliferation－and－Apoptosis－Methods
Chapter 1: Cell Death - Apoptosis and Necrosis1.1Introduction21.1.1Terminology of cell death21.1.2Differences between necrosis and apoptosis31.1.3Apop
Beginners－Guide:－Validat,－Verificat－and－Monitoring－of－Inspection－Equipment
February 28, 2017 -Validation, verification and routine performance monitoring are often used interchangeably, creating confusion within organizat
基因編輯鼠的構建－Guide－RNA設計和篩選
眾所周知，CRISPR/Cas9被業內譽為“基因剪刀”，它可以高效地實現靶基因的編輯，自問世以來就備受關注和青睞。CRISPR/Cas9系統是由CRISPR相關基因和Cas9組成，Cas9核酸酶會在向導RNA（Guide RNA, gRNA）的指引下，在完整基因組上的特定位點完成切割反應，同
Cas9/guideRNA遏制“病媒”蚊子傳播病毒
每當夏日將至，人們就開始陷入到被蚊子支配的恐懼之中，小小的蚊子不僅讓很多人飽受皮肉之苦，還會傳播瘧疾、登革熱、絲蟲病等危害性較強的傳染病。因此，別看人類處在食物鏈的頂層，可幾十年過去了，除了殺蟲劑策略，我們仍然沒有什么一勞永逸的辦法對付蚊子。隨著CRISPR 基因編輯革命的爆發，人們開始將目光聚
標準PCR
What's?PCR??(Michael Blaber's Lab)Illustrated introduction to usr/localious aspects of PCR technique. It's very valuable not only for thos
標準PCR
·?????????What's PCR??(Michael Blaber's Lab)Illustrated introduction to usr/localious aspects of PCR technique. It's very valuable not onl
標準樣品相關技術標準
我國環境標準樣品研制、生產和應用所遵循的技術標準主要包括： HJ/T 173-2005 環境標準樣品研復制技術規范 GB/T15000.2-1994/ISO Guide 30:1991標準樣品常用術語及定義 GB/T15000.3-1994標準樣品定值的一般原則與統計方法 G
Neutralizing－Bioassay－Protocols
Neutralizing Bioassay ProtocolsIntroductionAntibodies that block binding of cytokines to their specific receptors and neutralize their effects are cri
RNAi原理FLASH演示
How does RNAi work? Genetic and biochemical data indicate a possible two-step mechanism for RNA interference (RNAi): an initiation step and an effecto
Realspace－and－realtime－dynamics－of－CRISPRCas9－visualized－by－...（一）
Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopyAbstractThe ?CRISPR-associated endonuclease Cas9 bind
Transcriptome－Coexpression－Analysis－Using－ATTEDII－for－Integrated－...
Transcriptome coexpression analysis is an excellent tool for predicting the physiological functions of genes. It is based on the “guilt-by-associa
肽段
?·?????????Designing Your Peptide? (Genosys)·?????????Handling & Storage of Peptides?(Genosys)Two Dimensional Peptide Mapping?(Sefton Lab)??Synthesizi
EGel?－CloneWell－Agarose－Gels
實驗概要Instructions are ?provided below for using the E-Gel?CloneWell pre-cast agarose gels with ?the E-Gel? iBase? Power System. For detailed instructio
SGS木塑材料檢測服務
當前，木塑材料主要檢測產品標準主要為以下標準： ASTM D7031-04 Standard Guide for Evaluating Mechanical and Physical Properties of Wood-Plastic Composite Products 木塑復合材料
Cytokine－Bioassays
Cytokine BioassaysIntroductionBiological activity of cytokines and their concentrations are commonly measured by cellular proliferation of primary cel
如何制備標準物質和標準溶液
?我們將致力于討論ISO Guide 80《質量控制物質的內部準備指南》。這個實用指南是值得一讀的,其內容與每天的工作實踐都息息相關。此外,我們還關注了在使用儲備溶液配制工作溶液的過程中可能發生的一些錯誤。2014年8月,國際標準化組織(ISO)發布了題為“質量控制物質的內部準備指南(QCMs)”的
CDNA文庫
?CDNA文庫（主要內容如下）·?????????Construction of cDNA Library·?????????Construction of Genome DNA Library·?????????Library Screening??OthersConstruction of cD
Immunohistochemistry－on－paraffin－embedded－sections
主要試劑1. Neutral-buffered Formalin, 10% (NBF), 1 liter (Commercially available pre-prepared from many laboratory reagent suppliers).Double -distilled H
PCR引物
PCR Primer Design and Reaction Optimization?(Molecular Biology Techniques Manual)??PCR Primer Design?(Newman Lab)??PCR Primer Design?(Eppendorf)Detail
PCR引物
PCR?Primer Design and Reaction Optimization?(Molecular Biology Techniques Manual)??PCR Primer Design?(Newman Lab)??PCR Primer Design?(Eppendorf)Detail
基本無菌化技術
INTRODUCTIONThe regulations promulgated to implement the amended Animal Welfare Act require that all survival surgery be performed using aseptic proce
上海生科院利用CRISPR/Cpf1系統實現水稻多基因定點編輯
3月19日，《分子植物》（Molecular Plant）雜志在線發表了中國科學院上海生命科學研究院植物逆境生物學研究中心朱健康研究組題為Multiplex Gene Editing in Rice using the CRISPR-Cpf1 System 的研究論文。該工作在水稻中利用CRIS
張鋒：想用“魔剪”CRISPR治療人類疾病，沒那么簡單
7 月31日，Broad研究所的CRISPR先驅張鋒及其研究小組成員David Scott在《自然》子刊Nature Medicine上發表了題為“Implications of human genetic variation in CRISPR-based therapeutic genome
南京大學李江談對“背靠背”論文的理解與思考
“搶發”論文 在當前學科領域精細化，學術分工明確化的大語境下，多個科研團隊針對同一主題進行研究的情況并不少見，合作與競爭是團隊在發展過程中無法回避的挑戰。“時間賽”目前是同一領域不同團隊之間進行競爭的重要模式，這一路徑在自然科學領域中表現得分外明顯。隨著科技進步與競爭加劇，科研人員能否趕在第一
Reverse－Transfection－for－Gene－Function－Analysis
This guide describes a microarray-based system for the functional analysis in mammalian cells of many genes in parallel. Mammalian cells are cultured