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the BioComputing Hypertext CoursebookUS mirror - Italy mirror - Original Files in Germany.The following set of tutorial texts has been developed for the award-winning VSNS-BCD BioComputing Courses, aimed at a mixed audience of biologists, computer scientists, and students + researchers of related areas, on a late undergraduate / graduate level.Ch. 0. Introduction, by David L. Steffe......閱讀全文
The siRNA user guide (revised May 6, 2004) Selection of siRNA duplexes from the target mRNA sequenceUsing Drosophila melanogaster lysa
Selection of siRNA duplexes from the target mRNA sequenceUsing Drosophila melanogaster lysates (Tuschl et al. 1999), we have systematically analyzed t
An Introduction to BiocomputingVersion 2.01October 1996David Steffen, Ph.D.President, Biomedical Computing, Inc.6626 WestchesterHouston, Texas 77
實驗概要The ChargeSwitch? gDNA Purification Kits allow rapid and efficient purification of genomic DNA from small volumes of human blood. Afte
I. INTRODUCTION 引言 This guidance helps sponsors of investigational new drug applications (INDs) or applicants of new drug applications (NDAs),
BASIC THEORY AND USE OF GC-MSbyDr. Eugenia SobolevaContent1. Introduction.2. GC-MS syste
實驗概要Central neurons lose the ability for axonal regrowth during development and typically do not regenerate their axons following axotomy once the
For ionisation to take place at all, chemical reaction between the sample and the&nbs
實驗概要The iPrep? GeneCatcher? gDNA Blood Kit allows rapid and automated extraction of genomic DNA (gDNA) from human blood including archived
Microscopes in Cell BiologyIntroductionMicroscopy has a major role in the study of cells. From the very beginning, researchers have tried to develop w
AbstractTerahertz (THz) metasurfaces have been explored recently due to their properties such as low material loss and ease of fabrication
2.2 Interaction mating - large scaleWith a few modifications, the procedure described above can be used to test for interactions between a single prey
實驗概要The PureLink? Genomic DNA Purification Kit allows rapid and efficient purification of genomic DNA. The kit is designed to efficiently
Acknowledgements The organizer of the workshop acknowledges Dr. Murray Milford, Professor and Interim Head, and Dr. Mark Hussey, Professor and In
through a boundary conductance, h, on faces at z = +/- L.is controlled by the parameter hL/k, the Biot Number, where k is the thermal conductivity
I. Safety ProceduresA. ChemicalsA number of chemicals used in this laboratory are hazardous. All manufacturers of hazardous materials are require
實驗概要Directed differentiation of specific lineages has been a focal point in the field of human embryonic stem cell (hESC) research. Cell r
The onion root tip and the whitefish blastula remain as the standard introduction to the study of mitosis. The onion has easily observable chromosomes
Sapphire Window Development and Testing.The effort of this work was devoted to fabricating ultrathin strengthened sapphire disks and to accurately
EuthanasiaEuthanasia and mouse necropsies require prior IACUC approval. The mode of euthanasia should be chosen which minimizes pain or distress to th
3) 50 mm Disks. Four 50 mm diameter, 2 mm thick disks were specially polished by Meller Optics to try to obtain strengthening disks. A p
ObjectiveThe objective of the experiment is to determine the effects of ethanol exposure on the embryonic development of zebrafish through observation
實驗概要 This protocol utilizes the powerful guanidine isothiocyanate–phenol:chloroform extraction method which allows the
Although many protocols for tubulin preparation are available, the procedure described below is the simplest and highest yielding preparation I have d
Section 2.1Nondenaturing Polyacrylamide Gel Electrophoresis of ProteinsJohn M. Walker1. IntroductionSDS-PAGE (Section 2.2) is probably the most
The failure data is shown for the 12.5 mm diameter disks is shown in Fig. 12. The average failure pressure for the standard disks is 12 MPa, whe
Screening Kinase Phosphorylation Motifs Using Peptide LibrariesIsaac A. Manke and Michael B. YaffeCenter for Cancer Research, Massachusetts Institute
Preparing Lambda DNA1- Coliphage lambda DNA is a widely used vector for recombinant DNA. The middle third of its 48,000 bp contains no genes required
AbstractWhen more than one bait will be used to screen a single library, significant time and resources can be saved by performing the interactor hunt
Sequencing GelPreparing and Running a Sequencing Gel (6% Polyacrylamide/Urea) A. Preparation of Gel Solution 1) Weigh out 50 g of Urea into