Laser Capture Microdissection (LCM)
Introduction to LCM (BJMU)
Preparation, LCM and RNA/DNA extraction of Frozen Tissue Sections (NIH Laser Capture Microdissection Core Lab)
Preparation and LCM of Paraffin Embedded Tissue Sections (NIH Laser Capture Microdissection Core Lab)
DNA Extraction Protocol of LCM tissue and PCR for DNA analysis(NIH Laser Capture Microdissection Core Lab)
RNA Extraction Protocol of LCM tissue and RT-PCR for mRNA analysis (NIH Laser Capture Microdissection Core Lab)
Paraffin-Embedded Tissue for LCM (Arcturus)
Paraffin embedding is a process in which fixed (formalin or other fixative) is infiltrated and then blocked out in paraffin to stabilize it for long-term storage and easy sectioning...
H & E Staining Protocol for LCM (Arcturus)
De-Paraffinization of Tissue Sections (Arcturus)
Remove paraffin from section for tissue preparation such as nucleic acid extraction.
DNA Extraction From Paraffin Section (Arcturus)
This extraction method has been used for measuring loss of heterozygosity (LOH), dideoxy fingerprinting (DDF), clonality analysis (chromosome X inactivation) and direct sequencing of PCR* products for single base mutational analysis. Extractions are typically performed on 500-1000 captured cells.
RNA Extraction from Paraffin Section (NIH Protocol) (Arcturus)
A Protocol for extracting RNA from paraffin embedded tissue.
cDNA Library Construction
Detailed protocol for cDNA library construction from microdissected material.